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dopa2 medium  (Biogems International)


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    Structured Review

    Biogems International dopa2 medium
    Protocol for differentiation and encapsulation of DA neurons. (A) Protocol for expansion of DA neural progenitors and final differentiation of DA neurons. (B) Representative phase-contrast images of DOPA1, <t>DOPA2,</t> and DOPA3 cells. Scale bar: 100 μm. (C) Timeline for encapsulation within SAPNS. DA, dopaminergic; SAPNS, self-assembling peptide nanofiber scaffolds. Color images are available online.
    Dopa2 Medium, supplied by Biogems International, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dopa2 medium/product/Biogems International
    Average 91 stars, based on 1 article reviews
    dopa2 medium - by Bioz Stars, 2026-04
    91/100 stars

    Images

    1) Product Images from "Peptide-Based Scaffolds for the Culture and Transplantation of Human Dopaminergic Neurons"

    Article Title: Peptide-Based Scaffolds for the Culture and Transplantation of Human Dopaminergic Neurons

    Journal: Tissue Engineering. Part A

    doi: 10.1089/ten.tea.2019.0094

    Protocol for differentiation and encapsulation of DA neurons. (A) Protocol for expansion of DA neural progenitors and final differentiation of DA neurons. (B) Representative phase-contrast images of DOPA1, DOPA2, and DOPA3 cells. Scale bar: 100 μm. (C) Timeline for encapsulation within SAPNS. DA, dopaminergic; SAPNS, self-assembling peptide nanofiber scaffolds. Color images are available online.
    Figure Legend Snippet: Protocol for differentiation and encapsulation of DA neurons. (A) Protocol for expansion of DA neural progenitors and final differentiation of DA neurons. (B) Representative phase-contrast images of DOPA1, DOPA2, and DOPA3 cells. Scale bar: 100 μm. (C) Timeline for encapsulation within SAPNS. DA, dopaminergic; SAPNS, self-assembling peptide nanofiber scaffolds. Color images are available online.

    Techniques Used:

    Characterization of DA neural progenitors and DA neurons. (A) Immunocytochemical staining of the following markers: Nestin: neural stem/progenitor, Tuj1 (β-III tubulin): neuron, MAP2: mature neuron, TH: DA neuron, Ki-67: proliferative cells, Synapsin: synaptic vesicle, FOXA2: midbrain DA neuron, PITX3: midbrain DA neuron, En-1 (Engrailed-1): midbrain DA neuron, DAPI: nucleus. Scale bar: 50 μm. (B) qRT-PCR analysis comparing gene expression in 2D and 3D (SAPNS-encapsulated) DA neurons at day 22 of differentiation. Data are presented as log2 of the fold change in expression relative to DOPA2 neural progenitors, shown in a bar graph (left) and heat map (right). Error bars represent standard deviation. p > 0.05, Student's unpaired t-test. 2D, two-dimensional; 3D, three-dimensional; qRT-PCR, quantitative reverse transcription-polymerase chain reaction; TH, tyrosine hydroxylase. Color images are available online.
    Figure Legend Snippet: Characterization of DA neural progenitors and DA neurons. (A) Immunocytochemical staining of the following markers: Nestin: neural stem/progenitor, Tuj1 (β-III tubulin): neuron, MAP2: mature neuron, TH: DA neuron, Ki-67: proliferative cells, Synapsin: synaptic vesicle, FOXA2: midbrain DA neuron, PITX3: midbrain DA neuron, En-1 (Engrailed-1): midbrain DA neuron, DAPI: nucleus. Scale bar: 50 μm. (B) qRT-PCR analysis comparing gene expression in 2D and 3D (SAPNS-encapsulated) DA neurons at day 22 of differentiation. Data are presented as log2 of the fold change in expression relative to DOPA2 neural progenitors, shown in a bar graph (left) and heat map (right). Error bars represent standard deviation. p > 0.05, Student's unpaired t-test. 2D, two-dimensional; 3D, three-dimensional; qRT-PCR, quantitative reverse transcription-polymerase chain reaction; TH, tyrosine hydroxylase. Color images are available online.

    Techniques Used: Staining, Quantitative RT-PCR, Expressing, Standard Deviation, Reverse Transcription Polymerase Chain Reaction



    Similar Products

    dopa2 medium  (Biogems International)
    91
    Biogems International dopa2 medium
    Protocol for differentiation and encapsulation of DA neurons. (A) Protocol for expansion of DA neural progenitors and final differentiation of DA neurons. (B) Representative phase-contrast images of DOPA1, <t>DOPA2,</t> and DOPA3 cells. Scale bar: 100 μm. (C) Timeline for encapsulation within SAPNS. DA, dopaminergic; SAPNS, self-assembling peptide nanofiber scaffolds. Color images are available online.
    Dopa2 Medium, supplied by Biogems International, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dopa2 medium/product/Biogems International
    Average 91 stars, based on 1 article reviews
    dopa2 medium - by Bioz Stars, 2026-04
    91/100 stars
    		  Buy from Supplier

    Image Search Results


    Protocol for differentiation and encapsulation of DA neurons. (A) Protocol for expansion of DA neural progenitors and final differentiation of DA neurons. (B) Representative phase-contrast images of DOPA1, DOPA2, and DOPA3 cells. Scale bar: 100 μm. (C) Timeline for encapsulation within SAPNS. DA, dopaminergic; SAPNS, self-assembling peptide nanofiber scaffolds. Color images are available online.

    Journal: Tissue Engineering. Part A

    Article Title: Peptide-Based Scaffolds for the Culture and Transplantation of Human Dopaminergic Neurons

    doi: 10.1089/ten.tea.2019.0094

    Figure Lengend Snippet: Protocol for differentiation and encapsulation of DA neurons. (A) Protocol for expansion of DA neural progenitors and final differentiation of DA neurons. (B) Representative phase-contrast images of DOPA1, DOPA2, and DOPA3 cells. Scale bar: 100 μm. (C) Timeline for encapsulation within SAPNS. DA, dopaminergic; SAPNS, self-assembling peptide nanofiber scaffolds. Color images are available online.

    Article Snippet: Twenty-four hours later, neurospheres were plated onto a six-well tissue culture polystyrene (TCPS) dish (Eppendorf) ( Supplementary Figure S2 ) coated with 5 μg/mL laminin (MilliporeSigma), in DOPA2 medium (base medium supplemented with 0.5 μM SAG [9128694; Biogems]), 5 μM Y-27632, and 10 ng/mL of the following growth factors (PeproTech): stromal cell-derived factor 1-alpha (SDF-1α); Pleiotrophin; hepatocyte growth factor (HGF); insulin-like growth factor-2 (IGF-2); vascular endothelial growth factor-D (VEGF-D); secreted frizzled-related protein 1 (SFRP-1), fibroblast growth factor-8 (FGF-8); and 20 ng/mL cerebral dopamine neurotrophic factor (CDNF).

    Techniques:

    Characterization of DA neural progenitors and DA neurons. (A) Immunocytochemical staining of the following markers: Nestin: neural stem/progenitor, Tuj1 (β-III tubulin): neuron, MAP2: mature neuron, TH: DA neuron, Ki-67: proliferative cells, Synapsin: synaptic vesicle, FOXA2: midbrain DA neuron, PITX3: midbrain DA neuron, En-1 (Engrailed-1): midbrain DA neuron, DAPI: nucleus. Scale bar: 50 μm. (B) qRT-PCR analysis comparing gene expression in 2D and 3D (SAPNS-encapsulated) DA neurons at day 22 of differentiation. Data are presented as log2 of the fold change in expression relative to DOPA2 neural progenitors, shown in a bar graph (left) and heat map (right). Error bars represent standard deviation. p > 0.05, Student's unpaired t-test. 2D, two-dimensional; 3D, three-dimensional; qRT-PCR, quantitative reverse transcription-polymerase chain reaction; TH, tyrosine hydroxylase. Color images are available online.

    Journal: Tissue Engineering. Part A

    Article Title: Peptide-Based Scaffolds for the Culture and Transplantation of Human Dopaminergic Neurons

    doi: 10.1089/ten.tea.2019.0094

    Figure Lengend Snippet: Characterization of DA neural progenitors and DA neurons. (A) Immunocytochemical staining of the following markers: Nestin: neural stem/progenitor, Tuj1 (β-III tubulin): neuron, MAP2: mature neuron, TH: DA neuron, Ki-67: proliferative cells, Synapsin: synaptic vesicle, FOXA2: midbrain DA neuron, PITX3: midbrain DA neuron, En-1 (Engrailed-1): midbrain DA neuron, DAPI: nucleus. Scale bar: 50 μm. (B) qRT-PCR analysis comparing gene expression in 2D and 3D (SAPNS-encapsulated) DA neurons at day 22 of differentiation. Data are presented as log2 of the fold change in expression relative to DOPA2 neural progenitors, shown in a bar graph (left) and heat map (right). Error bars represent standard deviation. p > 0.05, Student's unpaired t-test. 2D, two-dimensional; 3D, three-dimensional; qRT-PCR, quantitative reverse transcription-polymerase chain reaction; TH, tyrosine hydroxylase. Color images are available online.

    Article Snippet: Twenty-four hours later, neurospheres were plated onto a six-well tissue culture polystyrene (TCPS) dish (Eppendorf) ( Supplementary Figure S2 ) coated with 5 μg/mL laminin (MilliporeSigma), in DOPA2 medium (base medium supplemented with 0.5 μM SAG [9128694; Biogems]), 5 μM Y-27632, and 10 ng/mL of the following growth factors (PeproTech): stromal cell-derived factor 1-alpha (SDF-1α); Pleiotrophin; hepatocyte growth factor (HGF); insulin-like growth factor-2 (IGF-2); vascular endothelial growth factor-D (VEGF-D); secreted frizzled-related protein 1 (SFRP-1), fibroblast growth factor-8 (FGF-8); and 20 ng/mL cerebral dopamine neurotrophic factor (CDNF).

    Techniques: Staining, Quantitative RT-PCR, Expressing, Standard Deviation, Reverse Transcription Polymerase Chain Reaction